Surface charge density is a basic quantity that is directly relevant to many phenomena, from surface interactions to DNA hybridization. Measurement and quantification of surface charge density can lead to a better understanding of biomolecular inter
Surface Plasmon Resonance (SPR) can be applied as a convenient, sensitive and label-free technique to study various surface phenomena. One such example is the Vroman effect exhibited by protein adsorption onto surfaces. This important effect arises
Endotoxin (commonly referred to as lipopolysaccharide in bacteriology) is associated with the outer membrane of Gram-negative bacterial pathogens such as Escherichia coli, Salmonella, Shigella, and Pseudomonas [1-2]. The interaction between endotoxi
In recent years, SPR has been used as an alternative detector for monitoring elution of a variety of species (e.g., polysaccharides and proteins [1-4]) out of liquid chromatographic (LC) columns. In addition to simplicity and fast speed the SPR dete
In restriction digestion, BSA has been used to stabilize enzymes during DNA digestion. It is also widely used as a biomolecule to block active sites on surfaces. Formation of the anti-BSA/BSA immune complex is relevant to studies of the receptor sit
SPR (SPR) has been demonstrated as a powerful technique for rapid, sensitive, and label-free genetic analysis [1-5]. When the sensor surface is coated with a single sensing (probe) DNA, SPR can be used for both affinity binding studies (i.e., kineti
SPR has been demonstrated to be a powerful optical technique for bioaffinity studies at the solid/solution interface. In general, SPR measures the change in refractive index originated from the binding of a solution species with a molecule pre-immob
Flow-injection SPR has been demonstrated as a viable alternative for sensitive detection of heavy metal ions at trace levels [1-4]. The impetus behind using SPR for elemental analysis stems from its high sensitivity, simplicity, compact design (for